Multiplex ELISA Kit For Mouse Cytokine Panel 2 (4-Plex)

96T/14000

IFN-γ, IL-1β, IL-6, TNFα

mouse

Serum, Plasma

Min 25 µl

5.5 hours

All analytes < 1% intra-panel cross-reactivity

Planar Based Multiplexed ELISA

96-well solid plate

The Multiplex Mouse Cytokine Panel 2 (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IFNγ, IL-1β, IL-6, TNFα).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 5.5 hours. Multiplex Arrays provide scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.

Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
IFNγ	Sandwich	516 - 0.71 (pg/mL)	361 (pg/mL)	0.94 (pg/mL)	0.35 (pg/mL)
IL-1β	Sandwich	2500 - 3.43 (pg/mL)	1750 (pg/mL)	4.5 (pg/mL)	1.71 (pg/mL)
IL-6	Sandwich	504 - 0.69 (pg/mL)	353 (pg/mL)	0.91 (pg/mL)	0.35 (pg/mL)
TNFα	Sandwich	429 - 0.59 (pg/mL)	300 (pg/mL)	0.69 (pg/mL)	0.29 (pg/mL)

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents Include:
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Substrate A: Liquid, 3.5 mL/vial
Substrate B: Liquid, 3.5 mL/vial
Sample Diluents: Liquid, 10 mL/vial of a buffered protein solution
Streptavidin HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Wash Buffer (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Plate Seals (3): Adhesive strips

Multiplex ELISA Kit For Mouse Cytokine Panel 2 (4-Plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK1016)