产品名称
Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex)
检测蛋白
IL-4, IL-6, IL-10, IFNγ
样本类型
Serum, EDTA/Heparin Plasma
分析物交叉反应
All analytes < 1% intra-panel cross-reactivity
实验类型
Planar Based Multiplexed ELISA
产品描述
The Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) is a fully quantitative ELISA-based chemiluminescent assay allowing concurrent measurement of four biomarkers or analytes (IL-4, IL-6, IL-10, IFNγ).
Each kit contains a 96-well plate, with each well featuring the relevant biomarker panel, and all the reagents required to perform testing. Multiplex plates are built by absorbing four distinct capture antibodies in a defined array to the bottom of each well. Our high-quality reagents help ensure the accuracy of your results.
Using just 25 µl of sample per well, up to 80 samples can be assayed for all four markers in the panel in 2.25 hours. Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) provides scientists with an easy-to-use and cost-effective means of generating a cytokine profile for each sample.
检测范围
Analyte | Assay Type | Calibrator Range | Upper Limit of Quantification (ULOQ) | Lower Limit of Quantification (LLOQ) | Limit of Detection |
---|
IFNγ | Sandwich | 99 – 0.14 (pg/mL) | 69.3 (pg/mL) | 0.18 (pg/mL) | 0.10 (pg/mL) |
IL-4 | Sandwich | 21 – 0.03 (pg/mL) | 14.7 (pg/mL) | 0.04 (pg/mL) | 0.02 (pg/mL) |
IL-6 | Sandwich | 333 – 0.46 (pg/mL) | 233 (pg/mL) | 0.59 (pg/mL) | 0.20 (pg/mL) |
IL-10 | Sandwich | 2680 – 3.68 (pg/mL) | 1876 (pg/mL) | 4.78 (pg/mL) | 2.98 (pg/mL) |
试剂盒内容
Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents include:
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Sample Diluent: Liquid, 10 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Detection Mix: Lyophilized, biotinylated antibodies in a buffered protein solution with preservatives. Reconstitute to 6 mL with Ultra Pure Lab Water
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Substrate A: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B+: Liquid, 3 mL/vial of stabilized signal enhancer
Plate Seals (3): Adhesive strips
文献引用格式
Multiplex ELISA Kit For Human Cytokine High Sensitivity (4-Plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK2006)