Multiplex ELISA Kit For Human Malaria (5-Plex)

96T/11000

CRP, HRP2, LDH-Pan, LDH-Pv, LDH-Pf

human

Whole Blood, Plasma, Serum

Min 15 µl

3.5 hours

All analytes < 1% intra-panel cross-reactivity

Planar Based Multiplexed ELISA

96-well solid plate

The Multiplex ELISA Kit For Human Malaria (5-Plex) is a robust and fully quantitative chemiluminescent assay for the concurrent surveillance of four malaria biomarkers. The assay simultaneously measures HRP2, LDH-Pan, LDH-Pv, LDH-Pf, and one host biomarker, CRP. The monitoring of subclinical Malaria infections and prevalence of HRP2-deletions is achieved by the coupling of the most sensitive commercially available HPR2 assay with LDH detection. Validated protocols for whole blood samples are in place, with protocols for dry blood spots in development.
Each kit contains a 96-well plate and all other reagents necessary to perform the assay. The individual wells of the plate are precisely and reproducibly arrayed with each of the five biomarkers for accurate results.
In 3.5 hours, each kit will report quantities of 80 samples for the four Malaria biomarkers and one host biomarker CRP. This is achieved while only using 15 μl of whole blood per sample.

Analyte	Assay Type	Calibrator Range	Upper Limit of Quantification (ULOQ)	Lower Limit of Quantification (LLOQ)	Limit of Detection
CRP	Competitive	62,800 – 61 (ng/mL)	11,125 (ng/mL)	60 (ng/mL)	30.5 (ng/mL)
HRP2	Sandwich	950 – 0.9 (pg/mL)	675 (pg/mL)	0.92 (pg/mL)	0.45 (pg/mL)
LDH -Pan	Sandwich	70,750 – 69 (pg/mL)	17,687.5 (pg/mL)	24 (pg/mL)	34.5 (pg/mL)
LDH-Pf	Sandwich	22,500 - 22 (pg/mL)	5,750 (pg/mL)	8 (pg/mL)	11 (pg/mL)
LDH-Pv	Sandwich	43,750 – 43 (pg/mL)	5,000 (pg/mL)	6 (pg/mL)	21.5 (pg/mL)

Each kit contains a 96-well plate (solid plate), with relevant biomarker panel in each well, and contains all reagents required to perform testing. If you want a strip-well format, please contact us for customization.
Reagents include:
Wash Buffer Concentrate (20X): Liquid, 50 mL/vial of a concentrated solution of buffered surfactant
Assay Diluent: Liquid, 6 mL/vial of a buffered protein solution with heterophilic antibody and rheumatoid factor blockers and preservatives
Calibrator Diluent: Lyophilized, buffered protein solution containing preservatives
Competitor: Lyophilized, biotinylated competitive antigen
Detection Mix: Liquid, 6 mL/vial of biotinylated antibodies in a buffered protein solution with preservatives
Calibrator: Lyophilized, recombinant antigens in a buffered protein base
Streptavidin-HRP (1X): Liquid, 6 mL/vial of streptavidin-conjugated horseradish peroxidase
Substrate A+: Liquid, 3 mL/vial of stabilized hydrogen peroxide
Substrate B: Liquid, 3 mL/vial of stabilized signal enhancer
Plate Seals (3): Adhesive strips

Multiplex ELISA Kit For Human Malaria (5-Plex) (Boster Biological Technology, Wuhan, China. Catalog#MEK2008)