Anti-APC1/ANAPC1 (Phospho-S355) Antibody

Rabbit polyclonal antibody

说明书

P03471

100μg ￥7188
货期： 3-4周

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Western blot analysis of phosphorylated APC1 expression in lysate from asynchronous cells (lane 1), lysate from cells treated with thymidine to synchronize cells at the G1/S boundary (lane 2) and lysate from cells treated with nocodazole to synchronize cells at the M phase (lane 3). Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Phosphorylated APC1 at 215KD was detected using rabbit anti-APC1 pS355 Antigen Affinity purified polyclonal antibody (Catalog # P03471) at 1:500. Data contributed by Bing Li, UT Southwestern.
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产品简介>

产品名称

Anti-APC1/ANAPC1 (Phospho-S355) Antibody

规格/价格

100μg/7188

指标别称

ANAPC1; APC1; Mitotic checkpoint regulator; TSG24

产品类型

Polyclonal

检验物种

human

应用范围

ELISA, IHC, WB

基因名称

ANAPC1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pS377 of Human Apc1 protein.

计算分子量

217 kDa

成分

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

纯化方式

This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. This antibody is specific for phosphorylated human APC1 protein at the pS377 residue. A BLAST analysis was used to suggest reactivity with this protein from human, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross-reactivity with APC1 protein from mouse is expected, as this sequence show 90% homologous between human and mouse. Cross-reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.

浓度

0.21 mg/mL by UV absorbance at 280 nm

产品形态

Liquid

保存条件

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

背景资料

APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Uniprot ID

Q9H1A4

文献引用格式

APC1/ANAPC1 (Phospho-S355) Antibody (Boster Biological Technology, Wuhan, China. Catalog#P03471)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Anaphase-promoting complex subunit 1

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实验步骤

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