Anti-AATF (Phospho-Ser477) Antibody

Rabbit polyclonal antibody

说明书

P03945

100μl ￥5580
货期： 3-4周

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser⁴⁷⁷ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
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产品简介>

产品名称

Anti-AATF (Phospho-Ser477) Antibody

规格/价格

100μl/5580

指标别称

AATF; CHE 1; CHE1; DED; Protein AATF; Rb binding protein Che 1; Traube

产品类型

Polyclonal

检验物种

human

应用范围

基因名称

AATF

抗体来源

Rabbit

抗体亚型

IgG

免疫原

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

计算分子量

63 kDa

成分

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

纯化方式

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

浓度

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

产品形态

Liquid

保存条件

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

背景资料

Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).

Uniprot ID

Q9NY61

文献引用格式

AATF (Phospho-Ser477) Antibody (Boster Biological Technology, Wuhan, China. Catalog#P03945)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Protein AATF

蛋白名全称

Protein AATF

实验方案>

实验步骤

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