Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:20-100 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of anti- ANXA4 antibody (PA1007-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat lung tissue lysates,
Lane 9: mouse lung tissue lysates.
Use rabbit anti- ANXA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ANXA4 at approximately 36KD. The expected band size for ANXA4 is at 36KD.
IHC analysis of Annexin A4/ANXA4 using anti-Annexin A4/ANXA4 antibody (PA1007-1).
Annexin A4/ANXA4 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-Annexin A4/ANXA4 Antibody (PA1007-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Annexin A4/ANXA4 using anti-Annexin A4/ANXA4 antibody (PA1007-1).
Annexin A4/ANXA4 was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-Annexin A4/ANXA4 Antibody (PA1007-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of anti- ANXA4 antibody (PA1007-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human PC-3 whole cell lysates,
Lane 8: rat lung tissue lysates,
Lane 9: mouse lung tissue lysates.
Use rabbit anti- ANXA4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ANXA4 at approximately 36KD. The expected band size for ANXA4 is at 36KD.
IHC analysis of Annexin A4/ANXA4 using anti-Annexin A4/ANXA4 antibody (PA1007-1).
Annexin A4/ANXA4 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-Annexin A4/ANXA4 Antibody (PA1007-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of Annexin A4/ANXA4 using anti-Annexin A4/ANXA4 antibody (PA1007-1).
Annexin A4/ANXA4 was detected in a paraffin-embedded section of rat pancreas tissue. The tissue section was incubated with rabbit anti-Annexin A4/ANXA4 Antibody (PA1007-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.