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Anti-NMDAR2A/GRIN2A Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC

PA1058-1

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of NMDAR2A/GRIN2A using anti-NMDAR2A/GRIN2A antibody (PA1058-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: rat brain tissue lysates,
    Lane 2: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NMDAR2A/GRIN2A antigen affinity purified polyclonal antibody (PA1058-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NMDAR2A/GRIN2A at approximately 180 kDa. The expected band size for NMDAR2A/GRIN2A is at 165 kDa.

    all(2)
  • IHC analysis of NMDAR2A/GRIN2A using anti-NMDAR2A/GRIN2A antibody (PA1058-1).
    NMDAR2A/GRIN2A was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-NMDAR2A/GRIN2A Antibody (PA1058-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

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产品名称
Anti-NMDAR2A/GRIN2A Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
GRIN2A; hNR2A; NMDAR2A/GRIN2A; NR2A
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC
基因名称
GRIN2A
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human NMDAR2A, different from the related mouse sequence by three amino acids, and from the related rat sequence by four amino acids.
计算分子量
165 kDa
实际分子量
180 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
GRIN2A is also known as N-methyl-D-aspartate receptor channel, subunit epsilon-1(NMDAR2A). This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants.
Uniprot ID
Q12879  
文献引用格式
NMDAR2A/GRIN2A Antibody (Boster Biological Technology, Wuhan, China. Catalog#PA1058-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:20-100
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-NMDAR2A/GRIN2A Antibody

    筛选器: All WB IHC

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