Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (PB0024).
Lane 1: recombinant Rat CCL3 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIP-1 Alpha/CCL3 antigen affinity purified polyclonal antibody (PB0024) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIP-1 Alpha/CCL3 at approximately 33 kDa.
Western blot analysis of MIP-1 Alpha/CCL3 using anti-MIP-1 Alpha/CCL3 antibody (PB0024).
Lane 1: recombinant Rat CCL3 Protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MIP-1 Alpha/CCL3 antigen affinity purified polyclonal antibody (PB0024) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MIP-1 Alpha/CCL3 at approximately 33 kDa.