Anti-RUNX2 Antibody

筛选器： All WB ICC/IF

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  Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human RUNX2 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB0171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 50KD. The expected band size for RUNX2 is at 57KD.

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  Figure 2. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysate,lane 2: A431 whole cell lysate,lane 3: K562 whole cell lysate,lane 4: JURKAT whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB0171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 57KD. The expected band size for RUNX2 is at 57KD.

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  Figure 3.ICC analysis using anti- RUNX2 antibody (PB0171).was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti- rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

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  Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: recombinant human RUNX2 protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB0171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 50KD. The expected band size for RUNX2 is at 57KD.

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  Figure 2. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB0171). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysate,lane 2: A431 whole cell lysate,lane 3: K562 whole cell lysate,lane 4: JURKAT whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB0171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 57KD. The expected band size for RUNX2 is at 57KD.

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  Figure 3.ICC analysis using anti- RUNX2 antibody (PB0171).was detected in immersion fixed A431 cell line . Cells were stained using the Dylight488-conjugated Anti- rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).