Western blot (WB): | 1:500-2000 |
Western blot analysis of SNAI1 using anti-SNAI1 antibody (PB0449). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human SMMC-7721 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAI1 antigen affinity purified polyclonal antibody (PB0449) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAI1 at approximately 29 kDa. The expected band size for SNAI1 is at 29 kDa.
Western blot analysis of SNAI1 using anti-SNAI1 antibody (PB0449). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human COLO-320 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human MCF-7 whole cell lysates,
Lane 6: human SMMC-7721 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SNAI1 antigen affinity purified polyclonal antibody (PB0449) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SNAI1 at approximately 29 kDa. The expected band size for SNAI1 is at 29 kDa.