Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- ATP1A1 antibody (PB0504).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human U87-MG whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human HEPG2 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: monkey COS-7 whole cell lysates,Lane 8: human K562 whole cell lysates.Use rabbit anti- ATP1A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP1A1 at approximately 100-113KD. The expected band size for ATP1A1 at 113KD.
Figure 1. Western blot analysis of anti- ATP1A1 antibody (PB0504).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human U87-MG whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human HEPG2 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: monkey COS-7 whole cell lysates,Lane 8: human K562 whole cell lysates.Use rabbit anti- ATP1A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP1A1 at approximately 100-113KD. The expected band size for ATP1A1 at 113KD.