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Anti-TRPV5 Antibody

Rabbit polyclonal antibody

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筛选器: All WB

PB0549

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of TRPV5 using anti-TRPV5 antibody (PB0549). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Pancreas Tissue Lysate,Lane 2: Rat Lung Tissue Lysate,Lane 3: Rat Intestine Tissue Lysate,Lane 4: SW620 Whole Cell Lysate,Lane 5: COLO320 Whole Cell Lysate,Lane 6: 293T Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # PB0549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83KD. The expected band size for TRPV5 is at 83KD.

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产品名称
Anti-TRPV5 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
Calcium transport protein 2; CAT2; ECaC; ECAC1; Epithelial calcium channel 1; Osm 9 like TRP channel 3; OTRPC3; TRPV5
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB
基因名称
TRPV5
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of human TRPV5 different from the related mouse and rat sequences by one amino acid.
计算分子量
83 kDa
实际分子量
83 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Transient receptor potential cation channel subfamily V member 5 is a protein that in humans is encoded by the TRPV5 gene. This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. And this protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. In addition, TRPV5 is mainly expressed in kidney epithelial cells, where it plays an important role in the reabsorption of Ca2+. Genetic deletion of TRPV5 in mice leads to Ca2+ loss in the urine, and consequential hyperparathyroidism, and bone loss.
Uniprot ID
Q9NQA5  
文献引用格式
TRPV5 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0549)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
transient receptor potential cation channel, subfamily V, member 5
蛋白名全称
Transient receptor potential cation channel subfamily V member 5
推荐稀释比
Western blot (WB):1:500-2000

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    Anti-TRPV5 Antibody

    筛选器: All WB

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