Anti-TSG101 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0550

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TSG101 using anti-TSG101 antibody (PB0550). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TSG101 antigA03957-Aen affinity purified polyclonal antibody (PB0550) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa.
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产品简介>

产品名称

Anti-TSG101 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; ESCRT I complex subunit TSG101; Exosome marker; TSG10; TSG101; tumor susceptibility gene 101; VPS23

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

TSG101

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TSG101 identical to the related mouse and rat sequences.

计算分子量

44 kDa

实际分子量

44 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TSG101, known as Tumor susceptibility gene 101, is mapped to 11p15. The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. And the protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Uniprot ID

Q99816

文献引用格式

TSG101 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0550)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tumor susceptibility 101

蛋白名全称

Tumor susceptibility gene 101 protein

实验方案>

实验步骤

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