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Anti-MMP9 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

PB0709

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of anti- MMP9 Antibody (PB0709). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: A549 whole cell lysates.
    Use rabbit anti- MMP9 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MMP9 at approximately 79KD. The expected band size for MMP9 is at 79KD.

    all(7)
  • Figure 2. IHC analysis using anti- MMP9 Antibody (PB0709). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(7)
  • Figure 3. IHC analysis using anti- MMP9 Antibody (PB0709). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(7)
  • Figure 4. IHC analysis using anti- MMP9 Antibody (PB0709). detected in paraffin-embedded section of human lymphadenoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(7)
  • Figure 5. IHC analysis using anti- MMP9 Antibody (PB0709). detected in paraffin-embedded section of humanLymph nodes of gastric adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(7)
  • Figure 6. IHC analysis using anti- MMP9 Antibody (PB0709). detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(7)

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产品名称
Anti-MMP9 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; Matrix metalloproteinase 9; MMP 9; MMP9; MMP-9; MMP9 (N-terminal)
产品类型
Polyclonal
检验物种
human
应用范围
WB,IHC,ELISA
基因名称
MMP9
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the C-terminus of rat MMP-9 different from the related human sequence by nineteen amino acids, and from the related mouse sequence by nine amino acids.
计算分子量
79 kDa
实际分子量
79 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Matrix metallopeptidase 9 (MMP-9), also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB), is an enzyme that in humans is encoded by the MMP9 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Uniprot ID
P50282  
文献引用格式
MMP9 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0709)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
蛋白名全称
Matrix metalloproteinase-9
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-MMP9 Antibody

    筛选器: All WB IHC ELISA

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