Anti-TRPM8 Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0882

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TRPM8 using anti-TRPM8 antibody (PB0882). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: 22RV1 whole cell lysates,
Lane 3: SW620 whole cell lysates,
Lane 4: A549 whole cell lysates,
Lane 5: A431 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRPM8 antigen affinity purified polyclonal antibody (PB0882) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TRPM8 at approximately 127 kDa. The expected band size for TRPM8 is at 128 kDa.
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产品简介>

产品名称

Anti-TRPM8 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

LTrpC 6; LTRPC6; Trp p8; TRPM8; TRPP8

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

TRPM8

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TRPM8 different from the related mouse sequence by four amino acids, and from the related rat sequence by two amino acids.

计算分子量

128 kDa

实际分子量

127 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Transient receptor potential cation channel subfamily M member 8 (TRPM8), also known as the cold and menthol receptor 1 (CMR1), is a protein that in humans is encoded by the TRPM8 gene. TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. The signal is conducted from primary afferents (type C- and A-delta) eventually leading to the sensation of cold and cold pain. The TRPM8 protein is expressed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin whereas WS-12 and CPS-369 are the most selective agonist of TRPM8.

Uniprot ID

Q7Z2W7

文献引用格式

TRPM8 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0882)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transient receptor potential cation channel, subfamily M, member 8

蛋白名全称

Transient receptor potential cation channel subfamily M member 8

实验方案>

实验步骤

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