Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of Collagen I expression in Lane 1: mouse NIH/3T3 whole cell lysates. Collagen I at 139-180KD was detected using rabbit anti-Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Collagen I was detected in paraffin-embedded sections of mouse lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Collagen I was detected in paraffin-embedded sections of rat lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Western blot analysis of Collagen I expression in Lane 1: mouse NIH/3T3 whole cell lysates. Collagen I at 139-180KD was detected using rabbit anti-Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Collagen I was detected in paraffin-embedded sections of mouse lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Collagen I was detected in paraffin-embedded sections of rat lung tissues using rabbit anti- Collagen I Antigen Affinity purified polyclonal antibody (Catalog # PB0981) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).