Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat RH-35 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse Hepa1-6 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Invasive urothelial carcinoma of the bladder with squamous differentiation. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Renal clear cell carcinoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Diffuse large B-cell lymphoma of the intestines tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human thyroid tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021) and anti-Beta Tubulin antibody (M01857-3).
YAP1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of YAP1 using anti-YAP1 antibody (PB1021) in HepG2 whole cell lysate.
Western blot analysis of YAP1 using anti- YAP1 antibody (PB1021).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- YAP1 antibody in HepG2 whole cell lysate,
Lane 3: anti- YAP1 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Flow Cytometry analysis of U251 cells using anti-YAP1 antibody (PB1021).
Overlay histogram showing U251 cells stained with PB1021 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB1021) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:250-300 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat RH-35 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse Hepa1-6 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Invasive urothelial carcinoma of the bladder with squamous differentiation. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Renal clear cell carcinoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Diffuse large B-cell lymphoma of the intestines tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human thyroid tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021) and anti-Beta Tubulin antibody (M01857-3).
YAP1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of YAP1 using anti-YAP1 antibody (PB1021) in HepG2 whole cell lysate.
Western blot analysis of YAP1 using anti- YAP1 antibody (PB1021).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- YAP1 antibody in HepG2 whole cell lysate,
Lane 3: anti- YAP1 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Flow Cytometry analysis of U251 cells using anti-YAP1 antibody (PB1021).
Overlay histogram showing U251 cells stained with PB1021 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB1021) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Western blot analysis of YAP1 using anti-YAP1 antibody (PB1021). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat RH-35 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse Hepa1-6 whole cell lysates,
Lane 4: mouse RM1 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Invasive urothelial carcinoma of the bladder with squamous differentiation. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Renal clear cell carcinoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human Diffuse large B-cell lymphoma of the intestines tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in a paraffin-embedded section of human thyroid tissue. The tissue section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021) and anti-Beta Tubulin antibody (M01857-3).
YAP1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.
IF analysis of YAP1 using anti-YAP1 antibody (PB1021).
YAP1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-YAP1 Antibody (PB1021) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
IP analysis of YAP1 using anti-YAP1 antibody (PB1021) in HepG2 whole cell lysate.
Western blot analysis of YAP1 using anti- YAP1 antibody (PB1021).
Lane 1: HepG2 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- YAP1 antibody in HepG2 whole cell lysate,
Lane 3: anti- YAP1 antibody (2μg) + HepG2 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- YAP1 antigen affinity purified polyclonal antibody (PB1021) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.
Flow Cytometry analysis of U251 cells using anti-YAP1 antibody (PB1021).
Overlay histogram showing U251 cells stained with PB1021 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB1021) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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