Anti-ADAR Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

PB1028

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ADAR using anti-ADAR antibody (PB1028). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: MCF-7 whole cell lysates,
Lane 4: HEPG2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ADAR antigen affinity purified polyclonal antibody (PB1028) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ADAR at approximately 110 kDa/150 kDa. The expected band size for ADAR is at 136 kDa.
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IHC analysis of ADAR using anti-ADAR antibody (PB1028).
ADAR was detected in a paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ADAR Antibody (PB1028) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of ADAR using anti-ADAR antibody (PB1028).
ADAR was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ADAR Antibody (PB1028) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(4)
Flow cytometry analysis of A431 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
all(4)

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产品简介>

产品名称

Anti-ADAR Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ADAR; ADAR1; adenosine deaminase; RNA; DRADA; DSH; DSRAD; G1P1; IFI 4; IFI4; K88dsRBP; p136

产品类型

Polyclonal

检验物种

human, mouse

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ADAR

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ADAR1 recombinant protein (Position: S128-Q346). Human ADAR1 shares 90.2% and 50.7% amino acid (aa) sequence identity with mouse and rat ADAR1, respectively.

计算分子量

136 kDa

实际分子量

110 kDa/150 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Double-stranded RNA-specific adenosine deaminase, also known as ADAR1, is an enzyme that in humans is encoded by the ADAR gene. It is mapped to 1q21.3. This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.

Uniprot ID

P55265

文献引用格式

ADAR Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB1028)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

adenosine deaminase, RNA-specific

蛋白名全称

Double-stranded RNA-specific adenosine deaminase

实验方案>

实验步骤

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