Anti-Beclin 1/BECN1 Antibody

筛选器： All WB ICC/IF IP FCM

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  Western blot analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human PC-3 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse NIH/3T3 whole cell lysates,
  Lane 8: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

• 

  Western blot analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human PC-3 whole cell lysates,
  Lane 4: rat C6 whole cell lysates,
  Lane 5: rat PC-12 whole cell lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

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  IF analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076).
  Beclin 1/BECN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Beclin 1/BECN1 Antibody (PB9076) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  IP analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076) in MCF-7 whole cell lysate.
  Western blot analysis of Beclin 1/BECN1 using anti- Beclin 1/BECN1 antibody (PB9076).
  Lane 1: MCF-7 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- Beclin 1/BECN1 antibody in MCF-7 whole cell lysate,
  Lane 3: anti- Beclin 1/BECN1 antibody (2μg) + MCF-7 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

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  Flow Cytometry analysis of Raji cells using anti-Beclin 1/BECN1 antibody (PB9076).
  Overlay histogram showing Raji cells stained with PB9076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beclin 1/BECN1 Antibody (PB9076) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: human MCF-7 whole cell lysates,
  Lane 4: human PC-3 whole cell lysates,
  Lane 5: rat C6 whole cell lysates,
  Lane 6: rat PC-12 whole cell lysates,
  Lane 7: mouse NIH/3T3 whole cell lysates,
  Lane 8: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

• 

  Western blot analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human 293T whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: human PC-3 whole cell lysates,
  Lane 4: rat C6 whole cell lysates,
  Lane 5: rat PC-12 whole cell lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates,
  Lane 7: mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

• 

  IF analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076).
  Beclin 1/BECN1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Beclin 1/BECN1 Antibody (PB9076) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  IP analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076) in MCF-7 whole cell lysate.
  Western blot analysis of Beclin 1/BECN1 using anti- Beclin 1/BECN1 antibody (PB9076).
  Lane 1: MCF-7 whole cell lysates(30ug),
  Lane 2: Rabbit control IgG instead of anti- Beclin 1/BECN1 antibody in MCF-7 whole cell lysate,
  Lane 3: anti- Beclin 1/BECN1 antibody (2μg) + MCF-7 whole cell lysate (500μg).
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa.

• 

  Flow Cytometry analysis of Raji cells using anti-Beclin 1/BECN1 antibody (PB9076).
  Overlay histogram showing Raji cells stained with PB9076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beclin 1/BECN1 Antibody (PB9076) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.