Anti-AQP1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM

PB9473

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AQP1 using anti-AQP1 antibody (PB9473). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: mouse kidney tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AQP1 antigen affinity purified polyclonal antibody (PB9473) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AQP1 at approximately 25, 35-38 kDa. The expected band size for AQP1 is at 29 kDa.
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IHC analysis of AQP1 using anti-AQP1 antibody (PB9473).
AQP1 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-AQP1 Antibody (PB9473) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of AQP1 using anti-AQP1 antibody (PB9473).
AQP1 was detected in a paraffin-embedded section of mouse kidney tissue. The tissue section was incubated with rabbit anti-AQP1 Antibody (PB9473) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of AQP1 using anti-AQP1 antibody (PB9473).
AQP1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP1 Antibody (PB9473) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of AQP1 using anti-AQP1 antibody (PB9473).
AQP1 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP1 Antibody (PB9473) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow Cytometry analysis of U2OS cells using anti-AQP1 antibody (PB9473).
Overlay histogram showing U2OS cells stained with PB9473 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP1 Antibody (PB9473) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-AQP1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AQP 1; AQP CHIP; AQP1; Aquaporin 1; Aquaporin CHIP; CHIP28; CO; Urine water channel

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM

基因名称

AQP1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 1, different from the related mouse and rat sequences by one amino acid.

计算分子量

29 kDa

实际分子量

25, 35-38 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Aquaporin 1 is a 28-kD integral protein thought at first to be a breakdown product of the Rh polypeptide but was later shown to be a unique molecule that is abundant in erythrocytes and renal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Uniprot ID

P29972

RRID

AB_3082131

文献引用格式

AQP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9473)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aquaporin 1

蛋白名全称

Aquaporin-1

实验方案>

实验步骤

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