Figure 1. Western blot analysis of anti- MTOR antibody (A00003-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human HELA whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: human A431 whole cell lysates,Lane 6: human Jurkat whole cell lysates, Use rabbit anti- MTOR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
all(3)
Figure 2. IHC analysis of MTOR using anti-MTOR antibody (A00003-2).MTOR was detected in paraffin-embedded section of human colon cancer tissues. anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(3) 
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| ELISA： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- MTOR antibody (A00003-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human HELA whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: human A431 whole cell lysates,Lane 6: human Jurkat whole cell lysates, Use rabbit anti- MTOR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
Figure 2. IHC analysis of MTOR using anti-MTOR antibody (A00003-2).MTOR was detected in paraffin-embedded section of human colon cancer tissues. anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. Western blot analysis of anti- MTOR antibody (A00003-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human HELA whole cell lysates,Lane 4: monkey COS-7 whole cell lysates,Lane 5: human A431 whole cell lysates,Lane 6: human Jurkat whole cell lysates, Use rabbit anti- MTOR 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for MTOR at approximately 289KD. The expected band size for MTOR is at 289KD.
Figure 2. IHC analysis of MTOR using anti-MTOR antibody (A00003-2).MTOR was detected in paraffin-embedded section of human colon cancer tissues. anti-MTOR Antibody (A00003-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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