Anti-mTOR Antibody

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF

BM4182

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-mTOR antibody (BM4182). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-mTOR antigen affinity purified monoclonal antibody (BM4182) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for mTOR at approximately 289 kDa. The expected band size for mTOR is at 289 kDa.
all(3)
Immunohistochemical analysis of paraffin-embedded rat liver, using mTOR Antibody.
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产品简介>

产品名称

Anti-mTOR Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

FLJ44809; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; MTOR; RAFT1; Rapamycin target protein 1; RAPT1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

MTOR

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human mTOR

计算分子量

289 kDa

实际分子量

289 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

The mammalian target of rapamycin (mTOR), also known as the mechanistic target of rapamycin and FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by theMTOR gene. The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Uniprot ID

P42345

文献引用格式

mTOR Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM4182)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

Serine/threonine-protein kinase mTOR

实验方案>

实验步骤

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