Anti-IGF1R Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ELISA

A00070

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of IGF1R using anti-IGF1R antibody (A00070). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IGF1R antigen affinity purified polyclonal antibody (A00070) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IGF1R at approximately 155 kDa. The expected band size for IGF1R is at 155 kDa.
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IHC analysis of IGF1R using anti-IGF1R antibody (A00070).
IGF1R was detected in a paraffin-embedded section of mouse small intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGF1R Antibody (A00070) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of IGF1R using anti-IGF1R antibody (A00070).
IGF1R was detected in a paraffin-embedded section of mouse kidney tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-IGF1R Antibody (A00070) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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产品简介>

产品名称

Anti-IGF1R Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD221; IGF I receptor; IGF1R; IGFIR; IGFR; JTK13

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC, ELISA

基因名称

Igf1r

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived mouse IGF1 Receptor recombinant protein (Position: E31-K257). Mouse IGF1 Receptor shares 96% and 100% amino acid (aa) sequence identity with human and rat IGF1 Receptor, respectively.

计算分子量

155 kDa

实际分子量

155 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IGF1R(Insulin-like Growth Factor 1 (IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, it was identified a regulatory subunit of phosphatidylinositol (PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.

Uniprot ID

Q60751

RRID

AB_3080926

文献引用格式

IGF1R Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00070)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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