Anti-IGF1R Antibody (Clone#OTI4C4)

Mouse monoclonal antibody

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筛选器： All FCM WB

M00070-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Living HEK293T cells transfected with either overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-IGF1R antibody, and then analyzed by flow cytometry (1:100).
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Flow cytometric analysis of living HepG2 cells, using anti-IGF1R antibody, (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:25).
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Flow cytometric analysis of living A549 cells, using anti-IGF1R antibody, (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:25).
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Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-IGF1R monoclonal antibody (1:500).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF1R (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF1R (Cat# M00070-2)(1:2000).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IGF1R Antibody (Clone#OTI4C4)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD221; IGF I receptor; IGF1R; IGFIR; IGFR; JTK13

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

FCM, WB

基因名称

IGF1R

克隆号

OTI4C4

宿主

Mouse

抗体亚型

IgG1

免疫原

Human recombinant protein fragment corresponding to amino acids 741-935 of human IGF1R(NP_000866) produced in Insect.

计算分子量

155 kDa

实际分子量

81 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

IGF1R(Insulin-like Growth Factor 1(IGF-1) Receptor) is a protein found on the surface of human cells. It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1(IGF-1) and by a related hormone called IGF-2. It belongs to the large class of tyrosine kinase receptors. The IGF1R gene is mapped on 15q26.3. IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Using a yeast 2-hybrid system, Dey et al.(1998) identified a regulatory subunit of phosphatidylinositol(PI) 3-kinase, PIK3R3, as a binding partner of IGF1R. Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1. In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter. Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation. Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines. IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin. Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.

Uniprot ID

P08069

文献引用格式

IGF1R Antibody (Clone#OTI4C4) (Boster Biological Technology, Wuhan, China. Catalog#M00070-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

insulin-like growth factor 1 receptor

蛋白名全称

Insulin-like growth factor 1 receptor

实验方案>

实验步骤

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