Anti-NOX2/CYBB Antibody

Rabbit polyclonal antibody

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筛选器： All WB ELISA

A00328

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of NOX2 using anti-NOX2 antibody (A00328). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG cell lysate,Lane 2: human Hela cell lysate,Lane 3: human HepG2 cell lysate. -NOX2 antigen affinity purified polyclonal antibody (Catalog # A00328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) A specific band was detected for NOX2 at approximately 65KD. The expected band size for NOX2 is at 65KD.
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产品简介>

产品名称

Anti-NOX2/CYBB Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

CYBB; Cytochrome b 245 heavy chain; NOX2; p91 PHOX

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB,ELISA

基因名称

CYBB

宿主

Rabbit

抗体亚型

IgG

免疫原

E. coli-derived human NOX2/gp91phox recombinant protein (Position: F416-D500).

计算分子量

65 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein. NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Uniprot ID

P04839

RRID

AB_3081059

文献引用格式

NOX2/CYBB Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00328)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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