Figure 1. Western blot analysis of anti- CYBB antibody (A00328-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human HEPG2 whole cell lysates,Lane 3: human U937 whole cell lysates. Use rabbit anti- CYBB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CYBB at approximately 65KD. The expected band size for CYBB at 65KD.
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Figure 2. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 6. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 7. ICC analysis using anti- CYBB antibody (A00328-3). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
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Figure 8. Flow cytometry analysis of THP-1 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- CYBB antibody (A00328-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human HEPG2 whole cell lysates,Lane 3: human U937 whole cell lysates. Use rabbit anti- CYBB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CYBB at approximately 65KD. The expected band size for CYBB at 65KD.
Figure 2. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. ICC analysis using anti- CYBB antibody (A00328-3). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of THP-1 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CYBB antibody (A00328-3).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human HEPG2 whole cell lysates,Lane 3: human U937 whole cell lysates. Use rabbit anti- CYBB 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CYBB at approximately 65KD. The expected band size for CYBB at 65KD.
Figure 2. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human bladder cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human gastric cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human testicular cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis using anti- CYBB antibody (A00328-3). detected in paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 7. ICC analysis using anti- CYBB antibody (A00328-3). was detected in immersion fixed A431 cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 8. Flow cytometry analysis of THP-1 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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