Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).
all(6) 
Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
all(6) Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(6) | Western blot (WB): | 1:500-2000 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)Catalog # BA1127) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
Figure 1. Western blot analysis of anti- CASP3(P17) Antibody (A00334-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Jurkat whole cell lysates,
Lane 2: Hela whole cell lysates,
Lane 3: 293T whole cell lysates,
Lane 4: HepG2 whole cell lysates.
Use rabbit anti- CASP3 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CASP3 at approximately 35KD. The expected band size for CASP3 is at 32KD.
Figure 2. ICC analysis using anti- CASP3(P17) Antibody (A00334-2). was detected in immersion fixed SIHA cell line. Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog # BA1127) and counterstained with DAPI (blue).
Figure 3. Flow cytometry analysis of CACO-2 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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