Anti-Caspase 3/CASP3 (p12) Antibody (Clone#GEA-3)

Rabbit monoclonal antibody

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筛选器： All WB IHC ICC/IF IP

BM4620

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-Caspase 3/CASP3 (p12) antibody (BM4620). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Caspase 3/CASP3 (p12) antigen affinity purified monoclonal antibody (BM4620) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Caspase 3/CASP3 (p12) at approximately 35 kDa. The expected band size for Caspase 3/CASP3 (p12) is at 32 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Caspase-3 p12 Antibody.
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Immunofluorescent analysis using the Antibody.
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Immunofluorescent analysis of HeLa cells, using Caspase-3 p12 Antibody.
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产品简介>

产品名称

Anti-Caspase 3/CASP3 (p12) Antibody (Clone#GEA-3)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Apopain; CASP 3; CASP3; Caspase 3; Caspase 3/p17; Caspase3; CPP 32; CPP32; CPP32B; Cysteine protease CPP32; Protein Yama; SCA 1; SREBP cleavage activity 1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF, IP

基因名称

CASP3

克隆号

GEA-3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human Caspase-3 p12

计算分子量

32 kDa

实际分子量

35 kDa,(cleaved)20/17/12 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Caspase 3 is a caspase protein which interacts with Survivin, XIAP, CFLAR, Caspase 8, HCLS1, Deleted in Colorectal Cancer, TRAF3 and GroEL. This gene which is located on 4q35 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. And the caspase-3 activation in heart failure sequentially cleaves SRF and generates a truncated SRF that appears to function as a dominant-negative transcription factor. Additionally, the caspase-3 influence on bone mineral density should be considered in any in vivo application of caspase-3 inhibitors to the treatment of human disease. In erythroid precursors undergoing terminal differentiation, Hsp70 prevents active CASP3 from cleaving GATA1 and inducing apoptosis.

Uniprot ID

P42574

文献引用格式

Caspase 3/CASP3 (p12) Antibody (Clone#GEA-3) (Boster Biological Technology, Wuhan, China. Catalog#BM4620)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Caspase-3

实验方案>

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