Anti-ATF4 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC

A00371-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

加入购物车立即购买

Figure 1. Western blot analysis of anti- ATF4 antibody (A00371-1). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human Hela whole cell lysates.
Use rabbit anti- ATF4 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for ATF4 at approximately 50KD. The expected band size for ATF4 is at 39KD.
all(3)
Figure 2. IHC analysis of ATF4 using anti-ATF4 antibody (A00371-1).ATF4 was detected in paraffin-embedded section of human ovary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(3)
Figure 3. IHC analysis of ATF4 using anti-ATF4 antibody (A00371-1).ATF4 was detected in paraffin-embedded section of human prostatic cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ATF4 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ATF4; CREB 2; CREB2; DNA binding protein TAXREB67; TAXREB67; TXREB

产品类型

Polyclonal

检验物种

human

应用范围

WB,IHC

基因名称

ATF4

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human ATF4, which shares 97.6% amino acid (aa) sequence identity with both mouse and rat ATF4.

计算分子量

39 kDa

实际分子量

50 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

ATF4, Activating Transcription Factor 4, is also known as CREB2. ATF4 belongs to the large ATF/CREB family of transcription factors which bind DNA via their basic region and dimerize via their leucine zipper domain to form a variety of homo- and heterodimers to regulate gene transcription. It is identified that members of this family share significant sequence similarity within a leucine zipper DNA-binding motif and an adjacent basic region. The ATF4 gene is mapped to chromosome 22. Unlike CREB, which activates transcription from CRE-containing promoters, CREB2 functions as a specific repressor of CRE-dependent transcription. The transcriptional repressor activity resides within the C-terminal leucine zipper and basic domain region of the CREB2 protein.

Uniprot ID

P18848

RRID

AB_3081088

文献引用格式

ATF4 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00371-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除