Anti-ATF4 Antibody

筛选器： All WB ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of anti-ATF4 antibody (A00371-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (A00371-4) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF4 at approximately 45-50 kDa. The expected band size for ATF4 is at 39 kDa.

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  Figure 2. IF analysis of ATF4 using anti-ATF4 antibody (A00371-4) and anti-Tubulin alpha antibody (M03989-3).
  ATF4 was detected in an immunocytochemical section of Hela cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-ATF4 antibody (A00371-4).
  Overlay histogram showing SiHa cells stained with A00371-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF4 Antibody (A00371-4, 1:100). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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  Figure 1. Western blot analysis of anti-ATF4 antibody (A00371-4). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human 293T whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (A00371-4) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ATF4 at approximately 45-50 kDa. The expected band size for ATF4 is at 39 kDa.

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  Figure 2. IF analysis of ATF4 using anti-ATF4 antibody (A00371-4) and anti-Tubulin alpha antibody (M03989-3).
  ATF4 was detected in an immunocytochemical section of Hela cells. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) (Catalog # BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.

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  Figure 3. Flow Cytometry analysis of SiHa cells using anti-ATF4 antibody (A00371-4).
  Overlay histogram showing SiHa cells stained with A00371-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF4 Antibody (A00371-4, 1:100). DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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