Western blot (WB): | 1:500-2000 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
Western blot analysis of INSR using anti-INSR antibody (A00447-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INSR antigen affinity purified polyclonal antibody (A00447-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for INSR at approximately 155 kDa. The expected band size for INSR is at 156 kDa.
Western blot analysis of INSR using anti-INSR antibody (A00447-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat pancreas tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: mouse pancreas tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INSR antigen affinity purified polyclonal antibody (A00447-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for INSR at approximately 155 kDa. The expected band size for INSR is at 156 kDa.