Anti-INSR Antibody

Rabbit polyclonal antibody

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筛选器： All WB

BA0951

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of INSR using anti-INSR antibody (BA0951). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates,
Lane 2: PANC whole cell lysates,
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-INSR antigen affinity purified polyclonal antibody (BA0951) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for INSR at approximately 155 kDa. The expected band size for INSR is at 156 kDa.
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产品名称

Anti-INSR Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD220; HHF5; INSR; IR

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

INSR

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Insulin Receptor, different from the related rat and mouse sequences by two amino acids.

计算分子量

156 kDa

实际分子量

155 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

INSR(INSULIN RECEPTOR) is a tetramer of 2 alpha and 2 beta subunits that are coded by a single gene and are joined by disulfide bonds, a mechanism parallel to that of its ligand, insulin. It belongs to the large class of tyrosine kinase receptors. The insulin receptor gene is mapped to 19p13.2. The insulin receptor mediates their activity by causing the addition of a phosphate group to particular tyrosines on certain proteins within a cell. The INSR gene spans more than 120 kb and has 22 exons. Functional studies of the INSR SNPs show no effect on mRNA levels or splicing in peripheral blood leukocytes or on binding of insulin to mononuclear cells.

Uniprot ID

P06213

文献引用格式

INSR Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0951)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

insulin receptor

蛋白名全称

Insulin receptor(IR)

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实验步骤

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