Anti-ZEB1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF FCM

A00548-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ZEB1 using anti-ZEB1 antibody (A00548-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ZEB1 antigen affinity purified polyclonal antibody (A00548-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ZEB1 at approximately 200 kDa. The expected band size for ZEB1 is at 124 kDa.
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IHC analysis of ZEB1 using anti-ZEB1 antibody (A00548-2).
ZEB1 was detected in a paraffin-embedded section of human melanoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ZEB1 Antibody (A00548-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of ZEB1 using anti-ZEB1 antibody (A00548-2).
ZEB1 was detected in a paraffin-embedded section of human glioma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ZEB1 Antibody (A00548-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of ZEB1 using anti-ZEB1 antibody (A00548-2).
ZEB1 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ZEB1 Antibody (A00548-2) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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Flow cytometry analysis of U2OS cell(1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介>

产品名称

Anti-ZEB1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AREB6; BZP; DELTA EF1; delta EF1; Negative regulator of IL2; NIL 2 A; NIL 2 A zinc finger protein; NIL 2A; NIL2A; TCF 8; TCF8; Transcription factor 8; ZEB; ZEB1; ZFHEP; ZFHX1A

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

ZEB1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human ZEB1, which shares 94.9% and 100% amino acid (aa) sequence identity with rat ZEB1.

计算分子量

124 kDa

实际分子量

200 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.

Uniprot ID

P37275

RRID

AB_3081167

文献引用格式

ZEB1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00548-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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