Western blot (WB): | 1:500-2000 |
Flow Cytometry (Fixed): | 1:50-200 |
Figure 1. Western blot analysis of SUR1 using anti-SUR1 antibody (A00895-1). Lane 1: human placenta tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates. anti-SUR1 antigen affinity purified polyclonal antibody (Catalog # A00895-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SUR1 at approximately 177KD. The expected band size for SUR1 is at 177KD.
Figure 2. Flow Cytometry analysis of A431 cells using anti-ABCC8 antibody (A00895-1).Overlay histogram showing A431 cells stained with A00895-1 (Blue line).anti-ABCC8 Antibody (A00895-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of SUR1 using anti-SUR1 antibody (A00895-1). Lane 1: human placenta tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates. anti-SUR1 antigen affinity purified polyclonal antibody (Catalog # A00895-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SUR1 at approximately 177KD. The expected band size for SUR1 is at 177KD.
Figure 2. Flow Cytometry analysis of A431 cells using anti-ABCC8 antibody (A00895-1).Overlay histogram showing A431 cells stained with A00895-1 (Blue line).anti-ABCC8 Antibody (A00895-1, 1:100) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.