Anti-nNOS/NOS1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB FCM ELISA

A01070-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- nNOS (neuronal)/NOS1 Antibody (A01070-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
Use rabbit anti- NOS1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for NOS1 at approximately 161KD. The expected band size for NOS1 is at 161KD.
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Figure 2. Flow cytometry analysis of U87 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-nNOS/NOS1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

bNOS; Constitutive NOS; IHPS1; N NOS; NC NOS; Neuronal NOS; Nitric oxide synthase; brain; nNOS; NOS; NOS type I; NOS1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, FCM, ELISA

基因名称

NOS1

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human nNOS (neuronal)/NOS1 recombinant protein (Position: R19-E1320).

计算分子量

161 kDa

实际分子量

161 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Nitric oxide synthase 1 (neuronal), also known as NOS1, is an enzyme that in humans is encoded by the NOS1 gene. The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

Uniprot ID

P29475

RRID

AB_3081337

文献引用格式

nNOS/NOS1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01070-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

nitric oxide synthase 1

蛋白名全称

Nitric oxide synthase, brain

实验方案>

实验步骤

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