Anti-nNOS/NOS1 Antibody (Clone#NOS-B1)

50μl/1180 100μl/1960 150μl/2780

bNOS; Constitutive NOS; IHPS1; N NOS; NC NOS; Neuronal NOS; Nitric oxide synthase; brain; nNOS; NOS; NOS type I; NOS1

Monoclonal

human, rat, goat, pig

WB

NOS1

NOS-B1

Mouse

IgG1

Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain.

161 kDa

161 kDa

200ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol.

Ascites

200ug/ml

Liquid

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal(Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.

P29475  

nNOS/NOS1 Antibody (Clone#NOS-B1) (Boster Biological Technology, Wuhan, China. Catalog#BM1311)

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.

nitric oxide synthase 1(neuronal)

Nitric oxide synthase, brain

Western blot (WB):

1:500-2000