Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
Flow Cytometry (Fixed): | 1:50-200 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Figure 1. Western blot analysis of NRF1 using anti-NRF1 antibody (A01129-2). Lane 1: human K562 whole cell lysate,Lane 2: human MDA-MB-231 whole cell lysate. anti-NRF1 antigen affinity purified polyclonal antibody (Catalog # A01129-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NRF1 at approximately 68KD. The expected band size for NRF1 is at 54KD.
Figure 2. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat heart tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human appendicitis tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 9. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human gastric cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 10. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human placenta tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 11. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human tonsil tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 12. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse cardiac muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 13. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 14. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat brain tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 15. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 16. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 17. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat lung tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 18. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat skeletal muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 19. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 20. Flow Cytometry analysis of A431 cells using anti- NRF1 antibody (A01129-2).
Overlay histogram showing A431 cells stained with A01129-2 (Blue line).anti- NRF1 Antibody (A01129-2,1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of NRF1 using anti-NRF1 antibody (A01129-2). Lane 1: human K562 whole cell lysate,Lane 2: human MDA-MB-231 whole cell lysate. anti-NRF1 antigen affinity purified polyclonal antibody (Catalog # A01129-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NRF1 at approximately 68KD. The expected band size for NRF1 is at 54KD.
Figure 2. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 6. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat heart tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 7. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 8. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human appendicitis tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 9. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human gastric cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 10. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human placenta tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 11. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human tonsil tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 12. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse cardiac muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 13. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 14. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat brain tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 15. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 16. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 17. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat lung tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 18. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat skeletal muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 19. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 20. Flow Cytometry analysis of A431 cells using anti- NRF1 antibody (A01129-2).
Overlay histogram showing A431 cells stained with A01129-2 (Blue line).anti- NRF1 Antibody (A01129-2,1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.