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Anti-NRF1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ICC/IF FCM ELISA

A01129-2

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 现货
  • Figure 1. Western blot analysis of NRF1 using anti-NRF1 antibody (A01129-2). Lane 1: human K562 whole cell lysate,Lane 2: human MDA-MB-231 whole cell lysate. anti-NRF1 antigen affinity purified polyclonal antibody (Catalog # A01129-2)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for NRF1 at approximately 68KD. The expected band size for NRF1 is at 54KD.

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  • Figure 2. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human ovary cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  • Figure 3. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  • Figure 4. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  • Figure 5. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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  • Figure 6. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat heart tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 7. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat kidney tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 8. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human appendicitis tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 9. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human gastric cancer tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 10. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human placenta tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 11. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of human tonsil tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 12. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse cardiac muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 13. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of mouse liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 14. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat brain tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 15. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat intestine tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 16. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat liver tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 17. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat lung tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 18. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat skeletal muscle tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 19. IHC analysis of NRF1 using anti-NRF1 antibody (A01129-2).NRF1 was detected in paraffin-embedded section of rat spleen tissue. anti-NRF1 Antibody (A01129-2) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

    all(22)
  • Figure 20. Flow Cytometry analysis of A431 cells using anti- NRF1 antibody (A01129-2).
    Overlay histogram showing A431 cells stained with A01129-2 (Blue line).anti- NRF1 Antibody (A01129-2,1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

    all(22)

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产品简介>

产品名称
Anti-NRF1 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
ALPHA PAL; NRF 1; NRF1; NRF1/nuclear respiratory factor 1; nuclear respiratory factor 1
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF, FCM, ELISA
基因名称
NRF1
宿主
Rabbit
抗体亚型
IgG
免疫原
E. coli-derived human NRF1 recombinant protein (Position: D246-Q503).
计算分子量
54 kDa
实际分子量
68 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Nuclear respiratory factor 1, is also known as NRF1. This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
Uniprot ID
Q16656  
文献引用格式
NRF1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01129-2)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Flow Cytometry (Fixed):1:50-200
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-NRF1 Antibody

    筛选器: All WB IHC ICC/IF FCM ELISA

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