Anti-Beta Actin/ACTB Antibody (HRP)

Rabbit polyclonal antibody

说明书

筛选器： All WB

A01263-HRP

100μl ￥880 200μl ￥1680
货期：现货

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Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (A01263-HRP). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (A01263-HRP). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.
all(2)
Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (A01263-HRP). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (A01263-HRP). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Beta Actin/ACTB Antibody (HRP)

规格/价格

100μl/880 200μl/1680

指标别称

ACTB; Beta-actin; B actin; Beta actin; PS1TP5BP1; β actin; BRWS1

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey, zebrafish, chicken

应用范围

基因名称

ACTB

抗体来源

Rabbit

抗体亚型

IgG

免疫原

HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

计算分子量

42 kDa

实际分子量

42 kDa

成分

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.

纯化方式

HRP-Beta-Actin antibody is affinity chromatography purified via peptide column.

浓度

0.5 mg/mL

产品形态

Liquid

保存条件

HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

背景资料

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Uniprot ID

P60709

文献引用格式

Beta Actin/ACTB Antibody (HRP) (Boster Biological Technology, Wuhan, China. Catalog#A01263-HRP)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Actin, beta

蛋白名全称

Actin, beta

实验方案>

实验步骤

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