Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- ACACA antibody (A01802-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- ACACA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACACA at approximately 266KD. The expected band size for ACACA is at 266KD.
Figure 1. Western blot analysis of anti- ACACA antibody (A01802-2). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: A549 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- ACACA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACACA at approximately 266KD. The expected band size for ACACA is at 266KD.