Anti-ACC1/ACACA Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB FCM ICC/IF ELISA

A01802-3

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- ACACA antibody (A01802-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human Jurkat whole cell lysate.
Use rabbit anti- ACACA 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ACACA at approximately 266KD. The expected band size for ACACA is at 266KD.
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Figure 3. Flow cytometry analysis of U251 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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Figure 2. IF analysis of ACACA using anti-ACACA antibody (A01802-3). ACACA was detected in an immunocytochemical section of U2OS cells. Dylight594-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1142) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-ACC1/ACACA Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

ACAC; ACACA; ACC; ACC1; ACCA; Acetyl CoA carboxylase 1

产品类型

Polyclonal

检验物种

human,rat

应用范围

WB, ICC/IF, FCM, ELISA

基因名称

ACACA

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human ACACA recombinant protein (Position: D31-R2336).

计算分子量

266 kDa

实际分子量

266 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Acetyl-CoA carboxylase 1 also known as ACC-alpha or ACCa is an enzyme that in humans is encoded by the ACACA gene. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Uniprot ID

Q13085

文献引用格式

ACC1/ACACA Antibody (Boster Biological Technology, Wuhan, China. Catalog#A01802-3)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

acetyl-CoA carboxylase alpha

蛋白名全称

Acetyl-CoA carboxylase 1

实验方案>

实验步骤

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