Anti-c-Rel/REL Antibody

筛选器： All WB ICC/IF FCM ELISA

• 

  Figure 1. Western blot analysis of anti-c-Rel/REL antibody (A01880-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates,
  Lane 2: human Daudi whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified polyclonal antibody (A01880-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.

• 

  Figure 2. IF analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1).
  c-Rel/REL was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Figure 3. Flow Cytometry analysis of U251 cells using anti-c-Rel/REL antibody (A01880-1).
  Overlay histogram showing U251 cells stained with A01880-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Rel/REL Antibody (A01880-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Figure 1. Western blot analysis of anti-c-Rel/REL antibody (A01880-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Raji whole cell lysates,
  Lane 2: human Daudi whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Rel/REL antigen affinity purified polyclonal antibody (A01880-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Rel/REL at approximately 78 kDa. The expected band size for c-Rel/REL is at 69 kDa.

• 

  Figure 2. IF analysis of c-Rel/REL using anti-c-Rel/REL antibody (A01880-1).
  c-Rel/REL was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Figure 3. Flow Cytometry analysis of U251 cells using anti-c-Rel/REL antibody (A01880-1).
  Overlay histogram showing U251 cells stained with A01880-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Rel/REL Antibody (A01880-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

•