Anti-Cyclin D1/CCND1 Antibody

筛选器： All WB FCM

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  Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (BA0770-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Rat Testis Tissue Lysate,
  Lane 2: Human Placenta Tissue Lysate,
  Lane 3: Rat Brain Tissue Lysate,
  Lane 4: MCF-7 Whole Cell Lysate,
  Lane 5: COLO320 Whole Cell Lysate,
  Lane 6: SW620 Whole Cell Lysate,
  Lane 7: MM231 Whole Cell Lysate.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # BA0770-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 34KD. The expected band size for Cyclin D1 is at 33KD.

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  Figure 2. Flow Cytometry analysis of U-87MG cells using anti- Cyclin D1 antibody (BA0770-2).
  Overlay histogram showing U-87MG cells stained with BA0770-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (BA0770-2, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Figure 1. Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (BA0770-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: Rat Testis Tissue Lysate,
  Lane 2: Human Placenta Tissue Lysate,
  Lane 3: Rat Brain Tissue Lysate,
  Lane 4: MCF-7 Whole Cell Lysate,
  Lane 5: COLO320 Whole Cell Lysate,
  Lane 6: SW620 Whole Cell Lysate,
  Lane 7: MM231 Whole Cell Lysate.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # BA0770-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 34KD. The expected band size for Cyclin D1 is at 33KD.

• 

  Figure 2. Flow Cytometry analysis of U-87MG cells using anti- Cyclin D1 antibody (BA0770-2).
  Overlay histogram showing U-87MG cells stained with BA0770-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (BA0770-2, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.