Anti-Cyclin D1/CCND1 Antibody (Clone#OTI1G2)

Mouse monoclonal antibody

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M00149-4

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期： 5-7天

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Figure 1. Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CCND1 monoclonal antibody (1:200).
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Figure 2. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCND1(Cat# M00149-4).
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Figure 3. Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-CCND1 monoclonal antibody. 1:500
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产品简介>

产品名称

Anti-Cyclin D1/CCND1 Antibody (Clone#OTI1G2)

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

B cell lymphoma 1 protein; CCND1; cyclin D; Cyclin D1; D11S287E; G1/S specific cyclin D1; PRAD1; PRAD1 oncogene; U21B31

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

基因名称

CCND1

克隆号

OTI1G2

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human CCND1 (NP_444284) produced in E.coli.

计算分子量

34 kDa

成分

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin induced IGF2 mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.

Uniprot ID

P24385

文献引用格式

Cyclin D1/CCND1 Antibody (Clone#OTI1G2) (Boster Biological Technology, Wuhan, China. Catalog#M00149-4)

应用释义

WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC－免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法

基因名全称

cyclin D1

蛋白名全称

G1/S-specific cyclin-D1

实验方案>

实验步骤

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