Anti-FAK/PTK2 Antibody

筛选器： All WB

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  Figure 1. Western blot analysis of FAK using anti-FAK antibody (BA0839-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human HEK293 whole cell lysates
  Lane 2: human PC-3 whole cell lysates
  Lane 3: human K562 whole cell lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK antigen affinity purified polyclonal antibody (Catalog # BA0839-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK at approximately 119KD. The expected band size for FAK is at 119KD.

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  Figure 2. Western blot analysis of FAK using anti-FAK antibody (BA0839-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: rat heart tissue lysates
  Lane 2: rat C6 whole cell lysates
  Lane 3: mouse RAW246.7 whole cell lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK antigen affinity purified polyclonal antibody (Catalog # BA0839-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK at approximately 119KD. The expected band size for FAK is at 119KD.

• 

  Figure 1. Western blot analysis of FAK using anti-FAK antibody (BA0839-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: human HEK293 whole cell lysates
  Lane 2: human PC-3 whole cell lysates
  Lane 3: human K562 whole cell lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK antigen affinity purified polyclonal antibody (Catalog # BA0839-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK at approximately 119KD. The expected band size for FAK is at 119KD.

• 

  Figure 2. Western blot analysis of FAK using anti-FAK antibody (BA0839-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  Lane 1: rat heart tissue lysates
  Lane 2: rat C6 whole cell lysates
  Lane 3: mouse RAW246.7 whole cell lysates
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK antigen affinity purified polyclonal antibody (Catalog # BA0839-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK at approximately 119KD. The expected band size for FAK is at 119KD.