| Western blot (WB): | 1:4000 |
| Immunohistochemistry (IHC): | 1:150 |
| Immunofluorescence (IF): | 1:100 |

Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and PTK2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTK2 monoclonal antibody M00151-1, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.

Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 4. Immunofluorescent staining of HeLa cells using anti-PTK2 mouse monoclonal antibody.

Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 6. Anti-PTK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK2 .

Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK2(Cat# M00151-1).

Figure 9. Western blot analysis of extracts (35ug) from 9 different cell by using anti-PTK2 monoclonal antibody (1:500).

Figure 1. Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and PTK2-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTK2 monoclonal antibody M00151-1, (1:100). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody ([M01692-2]) as a loading control.

Figure 2. Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 3. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 4. Immunofluorescent staining of HeLa cells using anti-PTK2 mouse monoclonal antibody.

Figure 5. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 6. Anti-PTK2 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK2 .

Figure 7. Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, M00151-1)

Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK2(Cat# M00151-1).

Figure 9. Western blot analysis of extracts (35ug) from 9 different cell by using anti-PTK2 monoclonal antibody (1:500).








