Anti-ERK1/MAPK3 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

BA1246

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Anti-ERK1/MAPK3 antibody, BA1246, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: Rat Skeletal Muscle Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: JURKAT Cell Lysate
Lane 7: RAJI Cell Lysate
Lane 8: COLO320 Cell Lysate
all(4)
Figure 2. IHC analysis of ERK1/MAPK3 using anti-ERK1/MAPK3 antibody (BA1246) .
ERK1/MAPK3 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-ERK1/MAPK3 Antibody (BA1246) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 3. IHC analysis of ERK1/MAPK3 using anti-ERK1/MAPK3 antibody (BA1246) .
ERK1/MAPK3 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-ERK1/MAPK3 Antibody (BA1246) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(4)
Figure 4. ICC analysis of ERK1/MAPK3 using anti-ERK1/MAPK3 antibody (BA1246).
ERK1/MAPK3 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ERK1/MAPK3 Antibody (BA1246) at a dilution of 1:100 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(4)

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产品简介>

产品名称

Anti-ERK1/MAPK3 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

MAP kinase 3; MAPK 3; ERT2; ERK-1; MAP kinase isoform p44; p44-MAPK; p44-ERK1; MAPK3; ERK1; PRKM3; HS44KDAP; HUMKER1A; P44ERK1; P44MAP; PRKM3

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB,IHC,ICC/IF

基因名称

MAPK3

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human ERK1, identical to the related mouse and rat sequences.

计算分子量

43 kDa

实际分子量

43 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.

Uniprot ID

P27361

文献引用格式

ERK1/MAPK3 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1246)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

mitogen-activated protein kinase 3

蛋白名全称

Mitogen-activated protein kinase 3(MAP kinase 3/MAPK 3)

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实验步骤

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