Western blot (WB): | 1:200~2000 |
Immunohistochemistry (IHC): | 1:150 |
Immunofluorescence (IF): | 1:100 |
Figure 1. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 4. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 7. Anti-MAPK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK3 .
Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK3 (Cat# M00104-5).
Figure 1. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 4. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)
Figure 7. Anti-MAPK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK3 .
Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK3 (Cat# M00104-5).