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Anti-ERK1/MAPK3 Antibody (Clone#OTI4D7)

Mouse monoclonal antibody

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M00104-5

  • 50μl ¥1180 100μl ¥1960 150μl ¥2780
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)

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  • Figure 2. Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MAPK3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

    all(8)
  • Figure 3. Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)

    all(8)
  • Figure 4. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)

    all(8)
  • Figure 5. Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)

    all(8)
  • Figure 6. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M00104-5)

    all(8)
  • Figure 7. Anti-MAPK3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAPK3 .

    all(8)
  • Figure 8. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLA-G (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK3 (Cat# M00104-5).

    all(8)

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产品名称
Anti-ERK1/MAPK3 Antibody (Clone#OTI4D7)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
MAP kinase 3; MAPK 3; ERT2; ERK-1; MAP kinase isoform p44; p44-MAPK; p44-ERK1; MAPK3; ERK1; PRKM3; HS44KDAP; HUMKER1A; P44ERK1; P44MAP; PRKM3
产品类型
Monoclonal
检验物种
human, monkey, mouse, rat, dog
应用范围
WB, IHC, IF
基因名称
MAPK3
克隆号
OTI4D7
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human MAPK3(NP_002737) produced in HEK293T cell.
计算分子量
43 kDa
实际分子量
43 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.
Uniprot ID
P27361  
文献引用格式
ERK1/MAPK3 Antibody (Clone#OTI4D7) (Boster Biological Technology, Wuhan, China. Catalog#M00104-5)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
mitogen-activated protein kinase 1; mitogen-activated protein kinase 3
蛋白名全称
Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3
推荐稀释比
Western blot (WB):1:200~2000
Immunohistochemistry (IHC):1:150
Immunofluorescence (IF):1:100

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