Anti-GAPDH Antibody

Rabbit Polyclonal Antibody

说明书

筛选器： All WB IHC

BA2913

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti-GAPDH antibody (BA2913). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human CCRF-CEM whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat smooth muscle tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (BA2913) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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Figure 2. Western blot analysis of anti-GAPDH antibody (BA2913). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: chicken heart tissue lysates,
Lane 2: chicken liver tissue lysates,
Lane 3: chicken brain tissue lysates,
Lane 4: monkey heart tissue lysates,
Lane 5: monkey lung tissue lysates,
Lane 6: monkey kidney tissue lysates,
Lane 7: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (BA2913) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
all(9)
Figure 3. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 4. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 5. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 6. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human testicular cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 7. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 8. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)
Figure 9. IHC analysis of GAPDH using anti-GAPDH antibody (BA2913).
GAPDH was detected in a paraffin-embedded section of rat brain tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
all(9)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GAPDH Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey, chicken

应用范围

WB, IHC

基因名称

GAPDH

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human GAPDH, identical to the related mouse and rat sequences.

计算分子量

36 kDa

实际分子量

36 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide(NAD). The enzyme is though to be a tetramer of identical chains. Several highly homologous glyceraldehyde-3-phosphate dehydrogenase(GAPD)-related sequences have been identified previously in human DNA by Southern blot analysis. Protein studies have identified only a single expressed locus for this major glycolytic enzyme, and this maps to chromosome 12p13. Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) is a critical regulator of CICD, it mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.

Uniprot ID

P04406

RRID

AB_2560936

文献引用格式

GAPDH Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2913)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Glyceraldehyde-3-phosphate dehydrogenase

实验方案>

实验步骤

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