产品名称
Anti-GAPDH Antibody(HRP conjugated)
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
G3PD; GAPD; GAPDH; OK/SW cl.12
检验物种
human, monkey, mouse, rat
免疫原
A synthesized peptide derived from human GAPDH(HRP conjugated)
成分
500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.
纯化方式
Affinity-chromatography
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
文献引用格式
GAPDH Antibody(HRP conjugated) (Boster Biological Technology, Wuhan, China. Catalog#BM3896)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
Glyceraldehyde-3-phosphate dehydrogenase
推荐稀释比
Western blot (WB): | 1:1000-5000 |