Western blot (WB): | 1:500-2000 |
Figure 1. Western blot analysis of anti- CCL1 antibody (BA3642-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SCG Whole Cell Lysates, Lane 2: COLO320 Whole Cell Lysates,Lane 3: JURKAT Whole Cell Lysates. Use rabbit anti- CCL1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCL1 at approximately 18KD. The expected band size for CCL1 is at 11KD.
Figure 1. Western blot analysis of anti- CCL1 antibody (BA3642-2).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: SCG Whole Cell Lysates, Lane 2: COLO320 Whole Cell Lysates,Lane 3: JURKAT Whole Cell Lysates. Use rabbit anti- CCL1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for CCL1 at approximately 18KD. The expected band size for CCL1 is at 11KD.